pE194 (ATCC® 37128)

Other IDs:

Nucleotide (GenBank) : VB0131 pE194 cloning vector.

 /  Applications: shuttle vector  /  Depositors: DM Ellis

Designations pE194
Permits and Restrictions

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Depositors DM Ellis
Other IDs

Nucleotide (GenBank) : VB0131 pE194 cloning vector.

Biosafety Level 1
Vector Information
Size (kb): 3.7
Vector: pE194 (plasmid)
Promoters: Promoter none
Construction: natural isolate
Construct size (kb): 3.7
Features: marker(s): eryR
promoter: none
replicon: pE194
terminator: none
enhancer: none
shuttle vector
Restriction digests of the clone give the following sizes (kb): AccI--3.8; PstI--3.8.
Temperature sensitive.
For full induction, the plasmid requires a period of induction by subinhibitory concentrations of erythromycin.
natural isolate from Staphylococcus aureus
confers resistance to macrolide, lincosamide, and streptogramin type B "MLS" antibiotics.
Media ATCC® Medium 1179: VY medium
Growth Conditions
Temperature: 30.0°C

Horinouchi S, Weisblum B. Posttranscriptional modification of mRNA conformation: mechanism that regulates erythromycin-induced resistance. Proc. Natl. Acad. Sci. USA 77: 7079-7083, 1980. PubMed: 6938954

Iordanescu S. Relationships between cotransducible plasmids in Staphylococcus aureus. J. Bacteriol. 129: 71-75, 1977. PubMed: 830649

Weisblum B, et al. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J. Bacteriol. 137: 635-643, 1979. PubMed: 104975

Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide and streptogramin type B antibiotics. J. Bacteriol. 150: 804-814, 1982. PubMed: 6279574

Shipped frozen