Aspergillus fumigatus Fresenius (ATCC® 96918)

Strain Designations: SRRC 2006 [CBS 286.95, IMI 370079, JCM 10253]  /  Product Format: freeze-dried

Strain Designations SRRC 2006 [CBS 286.95, IMI 370079, JCM 10253]
Application
Aspergillus reference strain for identification and morphological observation recommended by the International Commission on Penicillium and Aspergillus (ICPA)
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Genome Sequenced Strain

Yes

Comments
Aspergillus reference strain for identification and morphological observation recommended by the International Commission on Penicillium and Aspergillus (ICPA)

Genome sequencing strain (RIKEN Center for Life Science Technologies, Japan)
Morphology Colonies after 3-4 days on Blakeslee malt extract agar at 25°C conidia grey turquoise to dark turquoise to dark green and mycelium white. Conidial heads columnar, strips smooth-walled expanding gradually into pyriform. Conidia globose to broadly ovoid, smooth to finely roughened or spinose.
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCCAACCTCCCACCCGTGTCTATCGTACCTTGTTGCTTCGGCGGGCCCGCCGTTTCGACGGCCGCCGGGGAGGCCTTGCGCCCCCGGGCCCGCGCCCGCCGAAGACCCCAACATGAACGCTGTTCTGAAAGTATGCAGTCTGAGTTGATTATCGTAATCAGTTAAAACTTTCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCCCGTCCCCCTCTCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTTGTCACCTGCTCTGTAGGCCCGGCCGGCGCCAGCCGACACCCAACTTTATTTTTCTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA


D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCCCCTTCGGGGTCCGCGTTGTAATTTGCAGAGGATGCTTCGGGTGCAGCCCCCGTCTAAGTGCCCTGGAACGGGCCGTCATAGAGGGTGAGAATCCCGTCTGGGACGGGGTGTCTGCGTCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGTTTGCGACCAGACTCGCCCGCGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGTGGGCGGGCCAGCGTCGGTTTGGGCGGCCGGTCAAAGGCCCTCGGAATGTATCACCTCTCGGGGTGTCTTATAGCCGAGGGTGCAATGCGGCCTGCCTGGACCGAGGAACGCGCTTCGGCTCGGACGCTGGCGTAATGGTCGTAAATGAC


Beta-tubulin gene (bTub)

AGGTGTGTGGATGAAACTCTTGATTTATACTATTTCGGCAACATCTCACGATCTGACTCGCTACTAGGCCAACGGTGACAAATATGTTCCTCGTGCCGTTCTGGTCGATCTCGAGCCTGGTACCATGGACGCTGTCCGTGCCGGTCCCTTCGGCGAGCTATTCCGTCCCGACAACTTCGTCTTCGGCCAGTCCGGTGCTGGTAACAACTGG

Morphology Colonies after 3-4 days on Blakeslee malt extract agar at 25°C conidia grey turquoise to dark turquoise to dark green and mycelium white. Conidial heads columnar, strips smooth-walled expanding gradually into pyriform. Conidia globose to broadly ovoid, smooth to finely roughened or spinose.
Name of Depositor MA Klich
Cross References

Nucleotide (GenBank) : KC689328 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KF278478 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : BCGY00000000 Aspergillus fumigatus var. fumigatus strain JCM 10253, whole genome shotgun sequencing project

References

Klich MA, et al. Aspergillus reference cultures. New Orleans and Baarn: International Commission on Penicillium and Aspergillus; 1996.

Wang L, et al. Mitochondrial cytochrome b gene analysis of Aspergillus fumigatus and related species. J. Clin. Microbiol. 38: 1352-1358, 2000. PubMed: 10747106

Paoletti M, et al. Evidence for sexuality in the opportunistic fungal pathogen Aspergillus fumigatus. Curr. Biol. 15: 1242-1248, 2005. PubMed: 16005299

Hong SB, et al. Polyphasic taxonomy of Aspergillus fumigatus and related species. Mycologia 97: 1316-1329, 2005. PubMed: 16722222

Rydholm C, Szakacs G, Lutzoni F. Low genetic variation and no detectable population structure in Aspergillus fumigatus compared to closely related Neosartorya species. Eukaryot. Cell 5: 650-657, 2006. PubMed: 16607012

Mirhendi H, et al. Identification of pathogenic Aspergillus species by a PCR-restriction enzyme method. J. Med. Microbiol. 56: 1568-1570, 2007. PubMed: 17965363

Uemura N, et al. Development of a loop-mediated isothermal amplification method for diagnosing Pneumocystis pneumonia. J. Med. Microbiol. 57:50-57, 2008. PubMed: 18065667

Alshahni MM, et al. Direct colony PCR of several medically important fungi using Ampdirect plus. Jpn. J. Infect. Dis. 62(2): 164-167, 2009. PubMed: 19305063

Sato T, et al. Simple PCR-based DNA microarray system to identify human pathogenic fungi in skin. J. Clin. Microbiol. 48: 2357-2364, 2010. PubMed: 20421438

Nourmoradi H, et al. Ultraviolet irradiation: An effective inactivation method of Aspergillus spp. in water for the control of waterborne nosocomial aspergillosis. Water Res. 46: 5935-5940, 2012. PubMed: 22985523

Isshiki A, et al. Development of a multiple detection technique for fungi by DNA microarray with the simultaneous use of internal transcribed spacer region of ribosomal RNA gene and ╬▓-tubulin gene probes. Biocontrol. Sci. 19: 139-145, 2014. PubMed: 25252646

Diba K, et al. Development of RFLP-PCR method for the identification of medically important Aspergillus species using single restriction enzyme MwoI. Brazil J. Microbiol. 45:503-507, 2014. PubMed: 25242934