11B11 [BSF-1] (ATCC® HB-188)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody does not cross-react with other lymphokines, and can be used to purify BSF-1 from complex mixtures.

Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified BSF-1 emulsified in complete Freund's adjuvant.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine B cell stimulatory factor 1 (BSF-1, interleukin-4, interleukin 4, IL-4, B cell growth factor 1, BCGF-1)
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with L-glutamine, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
70% Culture Media + 20% FBS + 10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Isotype IgG1
Name of Depositor WE Paul
References

Ohara J, Paul WE. Production of a monoclonal antibody to and molecular characterization of B-cell stimulating factor-1. Nature 315: 333-336, 1985. PubMed: 2582266

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ohara J, Paul WE. Production of a monoclonal antibody to and molecular characterization of B-cell stimulating factor-1. Nature 315: 333-336, 1985. PubMed: 2582266