Aspergillus brasiliensis Varga et al. (ATCC® CRM-16404)

Strain Designations: WLRI 034(120) [CBS 733.88, DSM 1387, DSM 1988, IFO 9455, IMI 149007, NCPF 2275]  /  Product Format: freeze-dried

Deposited As Aspergillus niger van Tieghem, anamorph
Strain Designations WLRI 034(120) [CBS 733.88, DSM 1387, DSM 1988, IFO 9455, IMI 149007, NCPF 2275]
Application
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009
assay of antimicrobial preservatives
media testing
preparatory test control
quality control strain
sterility testing
testing
testing fungicides
transformation host
validation control
Antimicrobial effectiveness testing RefU.S. Pharmacopeia General Chapters:<51> ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011
Microbial enumeration testing RefU.S. Pharmacopeia General Chapters: <61> Microbial Examination of Nonsterile Products: Microbial Enumeration Tests. Rockville, MD USP34-NF29, 2011
This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified.
Biosafety Level 1
Product Format freeze-dried
Type Strain no
Intended Use

 

 

Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

 

    ACLASS Accredited Reference Material Producer AR-1384

Preceptrol® no
Comments Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

This strain was recently re-named as Aspergillus brasiliensis according to the reference Varga et al. 2007. 

Use of impedance for preservative efficacy testing

Morphology Colonies initially white or yellowish, mycelium growing rapidly (to cover plate in 5 to 10 days), soon producing dense layer of erect smooth-stiped, thick-walled conidiophores terminated by globose vesicles bearing phialides (uniseriate) or (commonly) metulae with phialides (biseriate) which produce dry chains of conidia. Revers of plate pale to grayish or greenish yellow, often showing radiating ridges in mycelium. Spore heads radiate, sometimes dividing into columns with age, initially pale, becoming dark brown to black. Individual conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) micrometers in diameter.
Morphology Colonies initially white or yellowish, mycelium growing rapidly (to cover plate in 5 to 10 days), soon producing dense layer of erect smooth-stiped, thick-walled conidiophores terminated by globose vesicles bearing phialides (uniseriate) or (commonly) metulae with phialides (biseriate) which produce dry chains of conidia. Revers of plate pale to grayish or greenish yellow, often showing radiating ridges in mycelium. Spore heads radiate, sometimes dividing into columns with age, initially pale, becoming dark brown to black. Individual conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) micrometers in diameter.
Name of Depositor SM Ringel
Isolation
Blueberry, North Carolina, USA
References

Connolly P, et al. The use of impedance for preservative efficacy testing of pharmaceuticals and cosmetic products. J. Appl. Bacteriol. 76: 68-74, 1994. PubMed: 8144407

Wnendt S, et al. Molecular cloning, sequence analysis and expression of the gene encoding an antifungal-protein from Aspergillus giganteus. Curr. Genet. 25: 519-523, 1994. PubMed: 8082203

ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA

British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 2003

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI C, 2003

Wnendt S, et al. Characterization of the gene encoding alpha-sarcin, a ribosome- inactivating protein secreted by Aspergillus giganteus. Gene 124: 239-244, 1993. PubMed: 8444347

Shen LL, et al. DNA topoisomerases from pathogenic fungi: targets for the discovery of antifungal drugs. Antimicrob. Agents Chemother. 36: 2778-2784, 1992. PubMed: 1336349

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U.S. Pharmacopeia General Chapters:<51> ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

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FF MicroPlate. Biolog.

Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10.

Sterility test, Growth promotion test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.54.

Preservatives-effectiveness test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.12.

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14698-1:2003.

Ophthalmic optics--Contact lens care products--Antimicrobial preservative efficacy testing and guidance on determining discard date. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14730:2000.

Chemical disinfectants and antiseptics -- Quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas -- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 1650:1998.

Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal, sporicidal and fungicidal activity. London, UK:British Standards Institution;British Standard BS EN 12353:2006.

Chemical disinfectants and antiseptics --- Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas --- Test method and requirements without mechanical action (phase 2/step 2). London, UK:British Standards Institution;British Standard BS EN 13697:2001.

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Chemical disinfectants and antiseptics --- Quantitative suspension test for the evaluation of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics --- Test method and requirements (phase 1). London, UK:British Standards Institution;British Standard BS EN 1275:2005.

Chemical disinfectants and antiseptics --- Quantitative carrier test for the evaluation of fungicidal or yeasticidal activity for instruments used in the medical area --- Test method and requirements (phase 2, step 2). London, UK:British Standards Institution;British Standard BS EN 14562:2006.

Chemical disinfectants and antiseptics --- Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in the veterinary area --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 1657:2005.

Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK:British Standards Institution;British Standard BS EN 14885:2006.

Cleanrooms and associated controlled environments --- Biocontamination control --- Part 1: General principles and methods. London, UK:British Standards Institution;British Standard BS EN ISO 14698:2003.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

Varga J, et al. Aspergillus brasiliensis sp. nov., a biseriate black Aspergillus species with world-wide distribution. Int. J. Syst. Evol. Microbiol. 57: 1925-1932, 2007.

U.S. Pharmacopeia General Chapters: <61> Microbial Examination of Nonsterile Products: Microbial Enumeration Tests. Rockville, MD USP34-NF29, 2011