Saccharomyces cerevisiae Hansen (ATCC® 20658)

Strain Designations: pYEGF8 [AB103]  /  Product Format: freeze-dried

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Strain Designations pYEGF8 [AB103]
produces protein epidermal growth factor urogastrone
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Type Strain no
Genotype MATalpha leu2-3 leu2-112 ura3-52 his4-580 pep4-3
Preceptrol® no
Mating Type alpha
Produces epidermal growth factor
Produces functional product (assayed by receptor binding) of 6 kDa, indicating proper excision of the alpha factor leader sequence.
The 1.75 kb insert consists of the gene for the alpha mating factor in which a central portion has been replaced by the synthetic sequence encoding epidermal growth factor.

The order of the major features in this plasmid is: BamHI - MATalpha/EGF/MATalpha - BamHI - 2 micron ori - LEU2 - 2 micron ori - pMB1 ori - bla.

DNA: Synthetic
Insert Lengths (kb): 1.75
Gene Product: epidermal growth factor (urogastrone) [EGF]
Alleles: A1, B1, B2, A2
Target Gene: epidermal growth factor
Medium ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Name of Depositor Chiron Corp., B Mendez, Chiron Corp.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.

Brake AJ. Secretory expression in eukaryotes. US Patent 4,870,008 dated Sep 26 1989

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation