Komagataella phaffii Kurtzman (ATCC® 20864)

Strain Designations: GS115 [SMD 83]  /  Product Format: frozen

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Deposited As Pichia pastoris (Guilliermond) Phaff
Strain Designations GS115 [SMD 83]
Transformation host
Transformation host for expression of beta-galactosidase
Transformation host for expression of epidermal growth factor
Transformation host for expression of alcohol oxidase
Transformation host for expression of recombinant Bordetella pertussis pertactin
Transformation host for expression of Bacillus stearothermophilus alanine carboxypeptidase gene, dacA
Transformation host for expression of HIV-1 envelope protein
Transformation host for expression of Geotrichum candidum lipase A and B
Transformation host for expression of recombinant human serum albumin
Transformation host for expression of recombinant human angiostatin
Transformation host for expression of synthetic tick anticoagulant peptide gene
Transformation host for expression of bovine enterokinase
Transformation host for expression of coffee bean alpha-galactosidase
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Genotype AOX1 his4-
Preceptrol® no
Genome Sequenced Strain


Ploidy haploid
Protein secretory pathway
Screening for muliple copy gene inserts
Komagataella pastoris was once used as a synonym for Pichia pastoris.
The sequences from the D1D2 region of LSU rDNA identified this strain as Komagataella phaffii.
Genome sequencing strain (Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, UGent, Belgium).
Medium ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 20°C to 25°C
Atmosphere: Typical aerobic
Sequenced Data
D1D2 region of the 26S ribosomal RNA gene


Name of Depositor SIBIA, Inc.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Mutant derived from NRRL Y-11430
Cross References

Nucleotide (GenBank) : KU729167 D1/D2 region of 26S rRNA gene


Buckholz RG. Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same. US Patent 5,268,273 dated Dec 7 1993

Vozza LA, et al. Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris. Bio-Technology 14: 77-81, 1996. PubMed: 9636316

Payne WE, et al. An inducible acid phosphatase from the yeast Pichia pastoris: characterization of the gene and its product. Gene 163: 19-26, 1995. PubMed: 7557473

Zhu A, et al. High-level expression and purification of coffee bean alpha- galactosidase produced in the yeast Pichia pastoris. Arch. Biochem. Biophys. 324: 65-70, 1995. PubMed: 7503561

Cregg JM, et al. Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5: 3376-3385, 1985. PubMed: 3915774

Laroche Y, et al. High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris. Bio-Technology 12: 1119-1124, 1994. PubMed: 7765555

Wagner LW, et al. Use of a silicone tubing sensor to control methanol concentration during fed batch fermentation of Pichia pastoris. Biotechnol. Tech. 11: 791-795, 1997.

Catoni E, et al. Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: high-level production and some properties of functional expressed lipase B. Biotechnol. Tech. 11: 689-695, 1997.

Fairlie WD, et al. Screening procedure for Pichia pastoris clones containing multiple copy gene inserts. BioTechniques 26: 1042-1044, 1999. PubMed: 10376135

Siegel RS, et al. Production of epidermal growth factor in Pichia pastoris yeast cells. US Patent 5,102,789 dated Apr 7 1992

de Hoop MJ, et al. Overexpression of alcohol oxidase in Pichia pastoris. FEBS Lett. 291: 299-302, 1991. PubMed: 1936277

Romanos MA, et al. Recombinant Bordetella pertussis pertactin (P69) from the yeast Pichia pastoris: high-level production and immunological properties. Vaccine 9: 901-906, 1991. PubMed: 1811375

Despreaux CW, Manning RF. The dacA gene of Bacillus stearothermophilus coding for D-alanine carboxypeptidase: cloning, structure and expression in Escherichia coli and Pichia pastoris. Gene 131: 35-41, 1993. PubMed: 8370539

Scorer CA, et al. The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene 136: 111-119, 1993. PubMed: 8293993

Kobayashi K, et al. High-level expression of recombinant human serum albumin from the methylotrophic yeast Pichia pastoris with minimal protease production and activation. J. Biosci. Bioeng. 89: 55-61, 2000.

Lin J, et al. Production process for recombinant human angiostatin in Pichia pastoris. J. Ind. Microbiol. Biotechnol. 24: 31-35, 2000.

Liu Y, et al. Preparation of high-activity whole cell biocatalysis by permeabilization of recombinant yeasts with alcohol. J. Biosci. Bioeng. 89: 554-558, 2000.

De Schutter K, et al. Genome sequence of the recombinant protein production host Pichia pastoris. Nat. Biotechnol. 27: 561-566, 2009. PubMed: 19465926

Salamin K, et al. Secretion of an endogenous subtilisin by Pichia pastoris strains GS115 and KM71. Appl Environ Microbiol 76: 4269-4276, 2010. PubMed: 20472730

Prabha L, et al. Identification of the dipeptidyl aminopeptidase responsible for N-terminal clipping of recombinant Exendin-4 precursor expressed in Pichia pastoris. Protein Expr Purif 64: 155-161, 2009. PubMed: 19028585

Zhang X, et al. Identification and characterization of a novel yeast omega3-fatty acid desaturase acting on long-chain n-6 fatty acid substrates from Pichia pastoris. Yeast 25: 21-27, 2008. PubMed: 17914745

Tanneberger K, et al. A novel form of 6-phosphofructokinase. Identification and functional relevance of a third type of subunit in Pichia pastoris. J. Biol. Chem. 282: 23687-23697, 2007. PubMed: 17522059

Miele R, Barra D, Bonaccorsi di Patti MC. A GATA-type transcription factor regulates expression of the high-affinity iron uptake system in the methylotrophic yeast Pichia pastoris. Arch Biochem Biophys 465: 172-179, 2007. PubMed: 17592720

Ternes P, et al. Identification of fungal sphingolipid C9-methyltransferases by phylogenetic profiling. J Biol Chem 281: 5582-5592, 2006. PubMed: 16339149

Bonaccorsi di Patti MC, et al. The yeast multicopper oxidase Fet3p and the iron permease Ftr1p physically interact. Biochem Biophys Res Commun 333: 432-437, 2005. PubMed: 15946650

Werten MWT, de Wolf FA. Reduced proteolysis of secreted gelatin and Yps1-mediated alpha-factor leader processing in a Pichia pastoris kex2 disruptant. Appl Environ Microbiol 71(5), 2310-2317, 2005. PubMed: 15870316

Bhatnagar A, et al. Yeast cytochrome c is a sequence-specific DNA-binding protein. Biochem Biophys Res Commun 321: 900-904, 2004. PubMed: 15358111

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation