Mucor circinelloides f. lusitanicus Schipper (ATCC® 1216B)

Strain Designations: [CBS 277.49, NRRL 3631]  /  Product Format: freeze-dried

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Deposited As Mucor racemosus Fresenius
Strain Designations [CBS 277.49, NRRL 3631]
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Genome Sequenced Strain

Yes

Mating Type minus
Comments
Translation rate
beta-Glucosidase synthesis
Dimorphism
Lipid synthesis
RNA synthesis
Mitochondrial genome
Construction of partial genomic libraries
Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA).
Morphology After 7 days at 25°C colony up to 13 mm in height, light gray; sporangiophores up to 6 (10) µm in diam., sympodially branched; sporangia at first pale yellowish then brownish black to black, up to 60 µm in diam., leaving small collars; columellae obovoid to globose, mouse-grey, up to 38 x 35 µm, infrequently larger; sporangiospores rather irregular in size and shape, ellipsoidal, a few globose, 4.4-6.1 (8) x 3.8-4.4 µm, chlamydospores and oidia in submerged mycelium; submerged mycelium containing yellow droplets.
Medium ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 323: Malt agar medium
ATCC® Medium 336: Potato dextrose agar (PDA)
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGAAGGATCATTAAATAATCAATAATTTTGGCTTGTCCATCATTATCTATTTACTGTGAAACGTATTATTACTTGACGCCTGAGGGATGTTCCACTGCTATAAGGATAGGCAGCGGAAATGTTAACCGAGTCATAATCAAGCTTAGGCTTGGTATCCTATTATTATTTACCAAAAGAATTCAGAATTAATATTGTAACATAGACGTAAAAAATCTATAAAACAACTTTTAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGTAGCAAAGTGCGATAACTAGTGTGAATTGCATATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCTCATTGGTATTCCAATGAGCACGCCTGTTTCAGTATCAAAACAAACCCTCTATCCAACTTTTGTTGAATAGGATGACTGAGAGTCTCTTGATCGTCAGATCTCGAACCTCTTGAAATGTACAAAGGCCTGATCTTGTTTGATGCCTGAACTTTTTTTTAATATAAAGAGAAGCTCTTGCGGTAAACTGTGCTGGGGCCTCCCAAATAACACTTTTTTAAATTTGATC


D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAAATAACAATGATTTCCCTAGTAACGGCGAGTGAAGAGGAAAGAGCTCAAAGTTGGAAACTGTTTGGCTTAGCTAAACCGTATTGTAAACTGTAGAAACATTTTCCTGGCACACCAGATTAATAAGTCCTTTGGAACAAGGCATCATGGAGGGTGAGAATCCCGTCTTTGATCTGAGTAGTTGTCTTTTGTGATATGTTTTCAAAGAGTCAGGTTGTTTGGGAATGCAGCCTAAATTGGGTGGTAAATCTCACCTAAAGCTAAATATTTGCGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGAACTTTGAAAAGAGAGTTAAACAGTATGTGAAATTGTTAAAAGGGAACCGTTTGGAGCCAGACTGGTTTGCTTGTAATCAACCTAGAATTCGTTTTGGGTGCACTTGCAGGCTATACCTGCCAACAACAGTTTGATTTGGAGGAAAAAATTAATAGGAATGTAGCCTCTCGAGGTGTTATAGCCTATTATCATACTCTGGATTGGACTGAGGAACGCAGCGAATGCCTTTAGGCAAGATTGCTGGGTGCTTTCGCTAATAAATGTTAGAATTTCTGCTTCGGGTGGTGCTAATGTTTAAAGGAGGAACACATCTAGTATATTTTTTATTCGCTTAGGTTGTTGGCTTAATGACTCTAAATGAC

Morphology After 7 days at 25°C colony up to 13 mm in height, light gray; sporangiophores up to 6 (10) µm in diam., sympodially branched; sporangia at first pale yellowish then brownish black to black, up to 60 µm in diam., leaving small collars; columellae obovoid to globose, mouse-grey, up to 38 x 35 µm, infrequently larger; sporangiospores rather irregular in size and shape, ellipsoidal, a few globose, 4.4-6.1 (8) x 3.8-4.4 µm, chlamydospores and oidia in submerged mycelium; submerged mycelium containing yellow droplets.
Name of Depositor AF Blakeslee
Cross References

Nucleotide (GenBank) : M26190 M.racemosus (Zygomycetes) 25S ribosomal RNA gene.

Nucleotide (GenBank) : X15582 Mucor racemosus RPG19 gene for ribosomal protein.

Nucleotide (GenBank) : X17475 Mucor racemosus TEF-3 gene for elongation factor 1-alpha

Nucleotide (GenBank) : X17476 Mucor racemosus TEF-2 gene for elongation factor 1-alpha

Nucleotide (GenBank) : J02605 M.racemosus TEF-1 gene encoding elongation factor 1-alpha

Nucleotide (GenBank) : M16353 M.racemosus elongation factor 1-alpha (TEF-2) gene, 3' end.

Nucleotide (GenBank) : M16354 M.racemosus elongation factor 1-alpha (TEF-3) gene, 3' end.

Nucleotide (GenBank) : M20232 M.racemosus elongation factor 1-alpha (TEF-2) gene, 5' end.

Nucleotide (GenBank) : M20233 M.racemosus elongation factor 1-alpha (TEF-3) gene, 5' end.

Nucleotide (GenBank) : U07696 Rhizomucor circinelloides f. lusitanicus ATCC 1216b putative

Nucleotide (GenBank) : AJ278321 Mucor racemosus partial gpd gene for glyceraldehyde-3-phosphate

References

Mukhtar M, et al. A procedure to construct partial genomic libraries for gene cloning from fungi. BioTechniques 17: 640-642, 1994. PubMed: 7833015

Orlowski M, Sypherd PS. Regulation of translation rate during morphogenesis in the fungus Mucor. Biochemistry 17: 569-575, 1978. PubMed: 623731

Schramke ML, Orlowski M. Mitochondrial genome of the dimorphic zygomycete Mucor racemosus. Curr. Genet. 24: 337-343, 1993. PubMed: 8252644

Orlowski M, Sypherd PS. RNA synthesis during morphogenesis of the fungus Mucor racemosus. Arch. Microbiol. 119: 145-152, 1978. PubMed: 727853

Borgia P, Sypherd PS. Control of beta-glucosidase synthesis in Mucor racemosus. J. Bacteriol. 130: 812-817, 1977. PubMed: 233721

Ito ET, et al. Lipid synthesis during morphogenesis of Mucor racemosus. J. Bacteriol. 152: 880-887, 1982. PubMed: 7130131

Inderlied CB, Sypherd PS. Glucose metabolism and dimorphism in Mucor. J. Bacteriol. 133: 1282-1286, 1978. PubMed: 641009

Nagy G, et al. Transcription of the three HMG-CoA reductase genes of Mucor circinelloides. BMC Microbiol 14: 93, 2014. PubMed: 24731286

Csernetics A, et al. Expression of three isoprenoid biosynthesis genes and their effects on the carotenoid production of the zygomycete Mucor circinelloides. Fungal Genet Biol 48: 696-703, 2011. PubMed: 21443966

Li CH, et al. Sporangiospore size dimorphism is linked to virulence of Mucor circinelloides. PLoS Pathog 7: e1002086, 2011. PubMed: 21698218

Lee SC, et al. Microsporidia evolved from ancestral sexual fungi. Curr Biol 18: 1675-1679, 2008. PubMed: 18976912

Nyilasi I, et al. High-affinity iron permease (FTR1) gene sequence-based molecular identification of clinically important Zygomycetes. Clin Microbiol Infect 14: 393-397, 2008. PubMed: 18190575

Velayos A, et al. Expression of the carG gene, encoding geranylgeranyl pyrophosphate synthase, is up-regulated by blue light in Mucor circinelloides. Curr Genet 43: 112-120, 2003. PubMed: 12695851

O'Donnell K, et al. Evolutionary relationships among mucoralean fungi (Zygomycota): Evidence for family polyphyly on a large scale. Mycologia 93: 286-296, 2001.

Voigt K, Wostemeyer J. Phylogeny and origin of 82 Zygomycetes from all 54 genera of the Mucorales and Mortierellales based on combined analysis of actin and translation elongation factor EF-1alpha genes. Gene 270: 113-120, 2001. PubMed: 11404008

Velayos A, et al. Blue-light regulation of phytoene dehydrogenase (carB) gene expression in Mucor circinelloides. Planta 210: 938-946, 2000. PubMed: 10872226

Velayos A, Eslava AP, Iturriaga EA. A bifunctional enzyme with lycopene cyclase and phytoene synthase activities is encoded by the carRP gene of Mucor circinelloides. Eur J Biochem 267: 5509-5519, 2000. PubMed: 10951210

Voigt K, Cigelnik E, O'donnell K. Phylogeny and PCR identification of clinically important Zygomycetes based on nuclear ribosomal-DNA sequence data. J Clin Microbiol 37: 3957-3964, 1999. PubMed: 10565914

Velayos A, et al. Interallelic complementation at the pyrF locus and the homodimeric nature of orotate phosphoribosyltransferase (OPRTase) in Mucor circinelloides. Mol Gen Genet 260: 251-260, 1998. PubMed: 9862479

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation