NCI-H2171 [H2171] (ATCC® CRL-5929™) Organism: Homo sapiens, human / Cell Type: lymphoblast / Tissue: lung; derived from metastatic site: pleural effusion / Disease: stage E, carcinoma; small cell lung cancer General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits View Restrictions Organism Homo sapiens, human Tissue lung; derived from metastatic site: pleural effusion Cell Type lymphoblast Product Format frozen Morphology floating clusters Culture Properties suspension Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease stage E, carcinoma; small cell lung cancer Age 50 years Gender male Ethnicity Caucasian Karyotype iso(3q) Derivation The line was established in April 1989 from a pleural effusion metastasis of a lung. Clinical Data 50 years Caucasian male The patient received prior chemotherapy and radiation therapy. The patient was a smoker. 150 pack years Comments A lymphoblastoid line from the same patient is available as ATCC CRL-5969. Complete Growth Medium HITES medium supplemented with 5% fetal bovine serumThe base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.) Cryopreservation Culture medium, 95%; DMSO, 5% Name of Depositor AF Gazdar, JD Minna Year of Origin 1989 References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS Restrictions The line is available with the following restrictions: This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. Any proposed commercial use of the these cells, or their products, must first be negotiated with the National Cancer Institute (NCI). For further information, please contact NCI’s Technology Transfer Center at NCI_TTC_Contact@mail.nih.gov or by phone at (240)-276-5514. References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.