2B8 (ATCC® CRL-12569)

Organism: Homo sapiens, human  /  Cell Type:: B lymphocyte; transfected with plasmid pSV2ne  /  Disease: Burkitt's lymphoma

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Organism Homo sapiens, human
Cell Type B lymphocyte; transfected with plasmid pSV2ne
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells contain EBV particles

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Burkitt's lymphoma
Age 7 years
Ethnicity Black, African
Applications
The 2B8 cell line (ATCC CRL-12569) is a HAT sensitive fusion cell line derived from the P3HR-1 Burkitt's lymphoma cell line (ATCC HTB-62).
Storage Conditions liquid nitrogen vapor temperature
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Derivation
The 2B8 cell line (ATCC CRL-12569) is a HAT sensitive fusion cell line derived from the P3HR-1 Burkitt's lymphoma cell line (ATCC HTB-62). P3HR1 cells harbor non-immortalizing EBV. P3HR-1 cells were cloned; treated with methanesulfonic acid ethyl ester (MSE) and plated in medium containing 6-thioguanine (6TG). They were selected for deficiency in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-). One of the clones was transfected with the neomycin resistance expression vector pSV2-neo to obtain the 2B8 cell line.
Clinical Data
Black, African
7 years
Comments
The 2B8 cell line (ATCC CRL-12569) is a HAT sensitive fusion cell line derived from the P3HR-1 Burkitt's lymphoma cell line (ATCC HTB-62). P3HR1 cells harbor non-immortalizing EBV. P3HR-1 cells were cloned; treated with methanesulfonic acid ethyl ester (MSE) and plated in medium containing 6-thioguanine (6TG). They were selected for deficiency in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-). One of the clones was transfected with the neomycin resistance expression vector pSV2-neo to obtain the 2B8 cell line.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation and subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell concentration between 1.0 X 10 exp5 and 1.0 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor Bayer Corporation
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000