BM-N (ATCC® CRL-8910)

Organism: Bombyx mori, silkworm  / 

Organism Bombyx mori, silkworm
Product Format frozen
Culture Properties mixed: adherent and suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
transfection host
Host suitable for infection by Bombyx mori nuclear polyhedrosis virus (BmNPV), and may be used to propagate BmNPV-based expression vectors.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
The line should be cultivated at 27C.
Complete Growth Medium The base medium for this cell line is IPL-41 Insect Cell Culture Medium with glutamine (available from Sigma or Gibco). To make the complete growth medium, add the following components to the base medium:
  • 2.6 g/L Tryptose Phosphate Broth (TPB)
  • 0.069 mg/L ZnSO4·7H2O
  • 3.86 mg/L AlCl3·6H2O
  • 5 mg/L chlorophenol red
  • 2g/L NaCl
  • 10% heat-inactivated insect grade fetal bovine serum

  • Adjust pH to 6.3 to 6.4 by adding 1N NaOH
    Adjust osmolarity to 317 to 360 by adding extra NaCl

    The IPL-41 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation. (In Vitro 17: 495-509, 1981)
Subculturing
Medium Renewal: Once per week
Gently resuspend attached cells in old culture medium by pipetting old medium across the cells on the floor of the flask.
Cryopreservation
Culture medium, 90%; DMSO, 10%
Culture Conditions
Temperature: 27.0°C
Name of Depositor Daiichi Seiyaku Co., Ltd.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References

Maeda S, et al. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature 315: 592-594, 1985. PubMed: 2989694

. . J. Seric. Sci. Jpn. 53: 547-548, 1984.

Maeda S, Furusawa M. Method of producing substances in living silkworms. US Patent 5,118,616 dated Jun 2 1992

Maeda S, et al. Method of producing peptides using baculovirus vectors in cultured cells. US Patent 5,110,729 dated May 5 1992

Basic Documentation
References

Maeda S, et al. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature 315: 592-594, 1985. PubMed: 2989694

. . J. Seric. Sci. Jpn. 53: 547-548, 1984.

Maeda S, Furusawa M. Method of producing substances in living silkworms. US Patent 5,118,616 dated Jun 2 1992

Maeda S, et al. Method of producing peptides using baculovirus vectors in cultured cells. US Patent 5,110,729 dated May 5 1992