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CMH1a (ATCC® CRL-8399™)

Organism: Mus musculus, mouse  /  Tissue: mammary gland  /  Disease: Papilloma, hepatitis

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Organism Mus musculus, mouse
Tissue mammary gland
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Papilloma, hepatitis
Strain RIII
Applications
This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.
HBsAg production is under the control of the metallothionine promoter.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.
HeLa Markers N
Genes Expressed
surface antigen [sAg]
Comments
This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.
HBsAg production is under the control of the metallothionine promoter.
The construct contains the entire BPV genome and the hepatitis B sAg structural gene inserted within the MT gene between the promoter and the rest of the gene.
A BamHI/SalI fragment of pRF300 (containing hepatitis B sAg and MT-1 promoter) was ligated to a BamHI/SalI fragment of pB2-2 (containing the entire BPV genome and pBR sequences) to create pRB-1.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:15 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature (or 37C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense, into new flasks.
Cryopreservation
fetal bovine serum, 90%; glycerol, 10%
Culture Conditions
Temperature: 37.0°C
Name of Depositor Integrated Genetics, Inc.
Deposited As Mus musculus
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References

Fitts RA, et al. Vector encoding hepatitis B surface antigen. US Patent 4,496,898 dated Sep 29 1987

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Fitts RA, et al. Vector encoding hepatitis B surface antigen. US Patent 4,496,898 dated Sep 29 1987