PWR-1E (ATCC® CRL-11611)

Organism: Homo sapiens, human  /  Cell Type: epithelial  /  Tissue: prostate  / 

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Organism Homo sapiens, human
Tissue
prostate
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2
[Cells contain Adenovirus 12 and SV40 DNA viral sequences]
Age 67 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype At passage 42, a majority of the cells were in the 56-59 range; XY RefWebber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420
Derivation Tumor Suppressor gene(s):p53 + [PubMed: 8761420] Human prostatic epithelial cells, derived from a normal prostate with mild hyperplasia, were immortalized with an adenovirus 12-SV40 hybrid virus (Ad12-SV40).

PWR-1E cell line (ATCC CRL-11611) was derived by single cell cloning of a non-producer cell [PubMed: 8761420].

 

Clinical Data
67 years
male
Antigen Expression
kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen)
Receptor Expression
androgen receptor, expressed
Genes Expressed
cytokeratin 18,cytokeratin 8,SV40 large T antigen,kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen)
Tumorigenic No
Effects
No, in nude mice (with or without Matrigel)
Yes, the cells do form colonies in soft agar
Comments Tumor Suppressor gene(s): p53 + [PubMed: 8761420] Human prostatic epithelial cells, derived from a normal prostate with mild hyperplasia, were immortalized with an adenovirus 12-SV40 hybrid virus (Ad12-SV40).

PWR-1E cells organize into acini and secrete PSA into the lumen, when exposed to androgen.

PWR-1E cells organize into acini and secrete PSA into the lumen, when exposed to androgen. When injected with Matrigel or with stromal cells, into athymic male rodents, PWR-1E cells also organize into acini and produce PSA [Webber MM, personal communication].

 

 

Complete Growth Medium The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit
  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
  • Subculturing Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS).
    3. Add 2.0 to 3.0 mL (to a T-25 flask) or 3.0 to 4.0 mL (to a T-75 flask) of 0.05% Trypsin - 0.53mM EDTA solution, diluted 1:1 with D-PBS, and place flask in a 37C incubator for 5 to 8 minutes. Observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
    4. Add 6.0 to 8.0 mL of 0.1% Soybean Trypsin Inhibitor (or 2% fetal bovine serum in D-PBS), as appropriate, and aspirate cells by gently pipetting.
    5. Transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 7 minutes.
    6. Discard supernatant and resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 2 X 104 to 4 X 104 viable cells/cm2 is recommended.
    7. Incubate cultures at 37°C. We recommend that you maintain cultures at a cell concentration between 4 X 104 and 7 X 104 cells/cm2.
    Cells grown under serum-free or reduced serum conditions may not attach strongly during the 24 hours after subculture and should be disturbed as little as possible during that period. 
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
    Medium Renewal: Every 2 days
    Cryopreservation
    Freeze Medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
    Storage Temperature: Liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.
    STR Profile
    Amelogenin: X,Y
    CSF1PO: 10,12
    D13S317: 12
    D16S539: 12,13
    D5S818: 11,13
    D7S820: 9,11
    THO1: 8,9.3
    TPOX: 8,11
    vWA: 14,16
    Isoenzymes
    AK-1, 1
    ES-D, 1
    G6PD, B
    GLO-I, 2
    Me-2, 0
    PGM1, 1
    PGM3, 1
    Name of Depositor Michigan State University, National Cancer Institute
    References

    Webber MM, Rhim JS. Human prostatic cell lines immortalized by adenovirus 12-simian virus 40 (AD12/SV40) hybrid virus. US Patent 5,610,043 dated Mar 11 1997

    Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420

    Okamoto M, et al. Interleukin-6 and epidermal growth factor promote anchorage-independent growth of immortalized human prostatic epithelial cells treated with N-methyl-N-nitrosourea. Prostate 35: 255-262, 1998. PubMed: 9609548

    Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636

    Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337

    Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152

    Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497

    upon exposure to androgen

    upregulated upon exposure to androgen

    Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Webber MM, Rhim JS. Human prostatic cell lines immortalized by adenovirus 12-simian virus 40 (AD12/SV40) hybrid virus. US Patent 5,610,043 dated Mar 11 1997

    Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420

    Okamoto M, et al. Interleukin-6 and epidermal growth factor promote anchorage-independent growth of immortalized human prostatic epithelial cells treated with N-methyl-N-nitrosourea. Prostate 35: 255-262, 1998. PubMed: 9609548

    Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636

    Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337

    Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152

    Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497

    upon exposure to androgen

    upregulated upon exposure to androgen

    Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420