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BB88 (ATCC® TIB-55™)

Organism: Mus musculus, mouse  /  Cell Type: erythroblast  /  Tissue: spleen  /  Disease: leukemia

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Organism Mus musculus, mouse
Tissue spleen
Cell Type erythroblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease leukemia
Strain BALB.B
Applications
This line was derived from a leukemia induced by B-tropic Friend murine leukemia virus.
The cells are negative for surface immunoglobulin (sIg-), Ia8 (Ia8-) and Thy-1.2 (Thy-1.2-).
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived from a leukemia induced by B-tropic Friend murine leukemia virus.
Antigen Expression
H-2b
Genes Expressed
globin mRNA; hemoglobin,H-2b
Cellular Products
globin mRNA; hemoglobin
Tumorigenic Yes
Effects
Yes, in BALB.B mice
Comments
This line was derived from a leukemia induced by B-tropic Friend murine leukemia virus.
The cells are negative for surface immunoglobulin (sIg-), Ia8 (Ia8-) and Thy-1.2 (Thy-1.2-).
Erythroid differentiation and hemoglobin synthesis are inducible by DMSO.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol and 10% heat-inactivated fetal bovine serum
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. The cells may also be grown as ascites in syngeneic mice. Inject 2 X 10 exp5 cell per mouse and harvest as soon as abdominal enlargement is observed (about 10 days).
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor B Chesebro
References

Chesebro B, et al. Lack of erythroid characteristics in Ia-positive leukemia cell lines induced by Friend murine leukemia virus: brief communication. J. Natl. Cancer Inst. 60: 239-242, 1978. PubMed: 564410

Chesebro B, et al. Characterization of Ia8 antigen, thy-1.2 antigen, complement receptors, and virus production in a group of murine virus-induced leukemia cell lines. J. Immunol. 117: 1267-1274, 1976. PubMed: 185295

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Chesebro B, et al. Lack of erythroid characteristics in Ia-positive leukemia cell lines induced by Friend murine leukemia virus: brief communication. J. Natl. Cancer Inst. 60: 239-242, 1978. PubMed: 564410

Chesebro B, et al. Characterization of Ia8 antigen, thy-1.2 antigen, complement receptors, and virus production in a group of murine virus-induced leukemia cell lines. J. Immunol. 117: 1267-1274, 1976. PubMed: 185295