GK1.5 (ATCC® TIB-207)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: Hybridoma: B lymphocyte  /  Tissue: Spleen  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue Spleen
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast-like
Culture Properties Suspension
Biosafety Level 1
Strain Lewis (B cell); BALB/c (myeloma)
Applications
The antibody profoundly blocks antigen specific murine class II MHC antigen reactive helper T lymphocyte lines.
Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with the cloned cytotoxic T lymphocyte lines V4 and 243/2.5.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against mouse helper, inducer T cells (L3T4 antigen, CD4)
Comments
L3T4 is expressed on mouse helper/inducer T cells and is analogous to the human Leu-3/T4 molecule.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 10cells/mL. Do not allow the cell concentration to exceed 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype Rat IgG2b
Name of Depositor FW Fitch
References

Fitch FW, et al. Evidence implicating L3T4 class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J. Immunol. 131: 2178-2183, 1983. PubMed: 6195255

Dialynas DP, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J. Immunol. 131: 2445-2451, 1983. PubMed: 6415170

Garvy BA, Harmsen AG. The role of T cells in infection-driven interstitial pneumonia after bone marrow transplantation in mice. Transplantation 62: 517-525, 1996. PubMed: 8781619

Murray HW, et al. Models of relapse of experimental visceral leishmaniasis. J. Infect. Dis. 173: 1041-1043, 1996. PubMed: 8603949

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902

Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838

Murray HW, et al. Multiple host defense defects in failure of C57BL/6 ep/ep (Pale Ear) mice to resolve visceral Leishmania donovani infection. Infect. Immun. 64: 161-166, 1996. PubMed: 8557335

Dialynas DP, et al. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol. Rev. 74: 29-56, 1983. PubMed: 6195085

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Fitch FW, et al. Evidence implicating L3T4 class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J. Immunol. 131: 2178-2183, 1983. PubMed: 6195255

Dialynas DP, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J. Immunol. 131: 2445-2451, 1983. PubMed: 6415170

Garvy BA, Harmsen AG. The role of T cells in infection-driven interstitial pneumonia after bone marrow transplantation in mice. Transplantation 62: 517-525, 1996. PubMed: 8781619

Murray HW, et al. Models of relapse of experimental visceral leishmaniasis. J. Infect. Dis. 173: 1041-1043, 1996. PubMed: 8603949

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902

Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838

Murray HW, et al. Multiple host defense defects in failure of C57BL/6 ep/ep (Pale Ear) mice to resolve visceral Leishmania donovani infection. Infect. Immun. 64: 161-166, 1996. PubMed: 8557335

Dialynas DP, et al. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol. Rev. 74: 29-56, 1983. PubMed: 6195085

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm