T3-3A1 [3A1] (ATCC® HB-2)

Organism: Mus musculus, mouse  /  Cell Type: hybridoma: B lymphocyte; somatic cell hybri  /  Disease: identifying T cell acute lymphoblastic leukemia.,identifying T cell acute lymphoblastic leukemia.

Permits and Restrictions

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Organism Mus musculus, mouse
Cell Type hybridoma: B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease identifying T cell acute lymphoblastic leukemia.,identifying T cell acute lymphoblastic leukemia.
Age adult
Strain

Strain:  BALB/c

Applications
The antibody recognizes a 40000 dalton cell surface antigen that is an early marker of the T cell lineage and may be useful for identifying T cell acute lymphoblastic leukemia.
This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22.
Comments
The antibody recognizes a 40000 dalton cell surface antigen that is an early marker of the T cell lineage and may be useful for identifying T cell acute lymphoblastic leukemia.
This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) cells/ml
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG1 kappa
Name of Depositor A Fauci, BF Haynes
References

Haynes BF, et al. Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets. Proc. Natl. Acad. Sci. USA 76: 5829-5833, 1979. PubMed: 316538

Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. PubMed: 6153676

Haynes BF, et al. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc. Natl. Acad. Sci. USA 77: 2914-2918, 1980. PubMed: 6156457

Prieyl JA, LeBien TW. Interleukin 7 independent development of human B cells. Proc. Natl. Acad. Sci. USA 93: 10348-10353, 1996. PubMed: 8816803

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Haynes BF, et al. Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets. Proc. Natl. Acad. Sci. USA 76: 5829-5833, 1979. PubMed: 316538

Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. PubMed: 6153676

Haynes BF, et al. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc. Natl. Acad. Sci. USA 77: 2914-2918, 1980. PubMed: 6156457

Prieyl JA, LeBien TW. Interleukin 7 independent development of human B cells. Proc. Natl. Acad. Sci. USA 93: 10348-10353, 1996. PubMed: 8816803