LBRM-33-1A5 (ATCC® CRL-8079)

Organism: Mus musculus, mouse  /  Tissue: spleen  /  Disease: lymphoma

Organism Mus musculus, mouse
Tissue spleen
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain B10.BR
Applications
The cells produce IL-2 in response to IL-1, and can be used to assay IL-1 activity.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Receptor Expression
interleukin 1 (IL-1)
Genes Expressed
interleukin-2 (interleukin 2, IL-2)
Cellular Products
interleukin-2 (interleukin 2, IL-2)
Comments
The cells produce IL-2 in response to IL-1, and can be used to assay IL-1 activity.
Complete Growth Medium RPMI 1640 medium with 0.0025 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Adherent cells can be dislodged by scraping. Maintain at approximately 8 X 10 exp5 viable cells/ml.
Name of Depositor S Gillis
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References

Gillis S. Process for preparing murine interleukin-2 in the presence of interleukin-1 from an interleukin-2 nonproducer malignant cell line and cell line therefor. US Patent 4,404,280 dated Sep 13 1983

Basic Documentation
References

Gillis S. Process for preparing murine interleukin-2 in the presence of interleukin-1 from an interleukin-2 nonproducer malignant cell line and cell line therefor. US Patent 4,404,280 dated Sep 13 1983