131-94H4 (ATCC® CRL-2739)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Age adult
Gender female
Strain 129GIX+
Applications
Protein is also detected in selected embryo tissues.
Animals were immunized with a synthetic polypeptide with the sequence RRKVEQEGYPQESSKAG(C) corresponding to peptides 119 to 135 of the MYB oncogene formerly v-myb (residues 2 to 18).
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with a synthetic polypeptide with the sequence RRKVEQEGYPQESSKAG(C) corresponding to peptides 119 to 135 of the MYB oncogene formerly v-myb (residues 2 to 18). Spleen cells were fused with Sp2/0 myeloma cells. Using immunoblot techniques, the antibody recognizes a 25 kd protein in BM2 cells and a protein of approximately 100 kd in Molt 4 cells. Protein is also detected in selected embryo tissues. The IgG1 and IgG2b reactivities of the antibody with the v-myb peptide (119 to 135) were confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly reactive.
Clinical Data
female
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic v-myb oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence RRKVEQEGYPQESSKAG(C) corresponding to peptides 119 to 135 of the MYB oncogene formerly v-myb (residues 2 to 18). Spleen cells were fused with Sp2/0 myeloma cells. Using immunoblot techniques, the antibody recognizes a 25 kd protein in BM2 cells and a protein of approximately 100 kd in Molt 4 cells. Protein is also detected in selected embryo tissues. The IgG1 and IgG2b reactivities of the antibody with the v-myb peptide (119 to 135) were confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly reactive.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 kappa
Name of Depositor National Cancer Institute
References

Animals were immunized with the synthetic peptide RRKVEQEGYPQESSKAG(C) corresponding to residues 119 through 135 of the MYB sequence (formerly residues 2 through 18 of the v-myb sequence).

Using immunoblot techniques, the antibody recognizes a 25 kd protein in BM2 cells and a protein of approximately 100 kd in Molt 4 cells. Protein is also detected in selected embryo tissues. The IgG1 and IgG2b reactivities of the antibody with the v-myb peptide (119 to 135) were confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly reactive.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Animals were immunized with the synthetic peptide RRKVEQEGYPQESSKAG(C) corresponding to residues 119 through 135 of the MYB sequence (formerly residues 2 through 18 of the v-myb sequence).

Using immunoblot techniques, the antibody recognizes a 25 kd protein in BM2 cells and a protein of approximately 100 kd in Molt 4 cells. Protein is also detected in selected embryo tissues. The IgG1 and IgG2b reactivities of the antibody with the v-myb peptide (119 to 135) were confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly reactive.