P-1 [P1] (ATCC® CRL-2588)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain BALB/c
Applications
The antibody reacts with the small subunit of bovine mu-caplain and m-calpain.
It cross-reacts with the small subunit of human and canine mu-caplain and m-calpain.
The antibody can be used for immunochemical and Western blot applications.
It is a useful tool for detecting and localizing the small subunit of calpain.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified bovine myocardial calpain 2
Spleen cells were fused with FOX-NY myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the small subunit of calpain
Cellular Products
immunoglobulin; monoclonal antibody; against the small subunit of calpain
Comments
Animals were immunized with purified bovine myocardial calpain 2
Spleen cells were fused with FOX-NY myeloma cells.
The antibody reacts with the small subunit of bovine mu-caplain and m-calpain.
It cross-reacts with the small subunit of human and canine mu-caplain and m-calpain.
The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the small subunit of calpain.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(4) viable cells/ml.
Interval: Maintain cell concentration between 3 X 10(4) and 3 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 kappa , IgG1; kappa light chain
Population Doubling Time 24 hours
Name of Depositor R Lane, RL Mellgren
Year of Origin 1986
References

Mellgren RL, Lane RD. Myocardial calpain 2 is inhibited by monoclonal antibodies specific for the small, noncatalytic subunit. Biochim. Biophys. Acta 954: 154-160, 1988. PubMed: 2835105

Zhang W, Mellgren RL. Calpain Subunits Remain Associated during Catalysis. Biochem. Biophys. Res. Commun. 227: 890-896, 1996. PubMed: 8954990

Zhang W, et al. The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J. Biol. Chem. 271: 18825-18830, 1996. PubMed: 8702541

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mellgren RL, Lane RD. Myocardial calpain 2 is inhibited by monoclonal antibodies specific for the small, noncatalytic subunit. Biochim. Biophys. Acta 954: 154-160, 1988. PubMed: 2835105

Zhang W, Mellgren RL. Calpain Subunits Remain Associated during Catalysis. Biochem. Biophys. Res. Commun. 227: 890-896, 1996. PubMed: 8954990

Zhang W, et al. The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J. Biol. Chem. 271: 18825-18830, 1996. PubMed: 8702541