VSW (ATCC® CCL-129)

Organism: Vipera russelli, Russell's viper  / 

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Organism Vipera russelli, Russell's viper
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Gender female
Applications
The cells produce a C type retrovirus.
Karyotype model number = 64; range = 33 to 118. Karyotype slightly unstable with stemline number in hypertriploid range. Cells with modal numbers of 28 and 29 macrochromosomes and 34 to 37 microchromosomes. Many cells with a very small chromosome with a median centromere included with macrochromosomes. All cells have 2 to 3 marker chromosomes with terminal centromeres.
Clinical Data
female
Comments
The cells produce a C type retrovirus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Once per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 30.0°C
Name of Depositor HF Clark
Year of Origin 1967
References

Zeigel RF, Clark HF. Electron microscopic observations on a "C"-type virus in cell cultures derived from a tumor-bearing viper. J. Natl. Cancer Inst. 43: 1097-1102, 1969. PubMed: 4982059

Zeigel RF, Clark HF. Histologic and electron microscopic observations on a tumor-bearing viper: establishment of a "C"-type virus-producing cell line. J. Natl. Cancer Inst. 46: 309-321, 1971. PubMed: 5165585

Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit

References

Zeigel RF, Clark HF. Electron microscopic observations on a "C"-type virus in cell cultures derived from a tumor-bearing viper. J. Natl. Cancer Inst. 43: 1097-1102, 1969. PubMed: 4982059

Zeigel RF, Clark HF. Histologic and electron microscopic observations on a tumor-bearing viper: establishment of a "C"-type virus-producing cell line. J. Natl. Cancer Inst. 46: 309-321, 1971. PubMed: 5165585

Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139