Malme-3 (ATCC® HTB-102™) Organism: Homo sapiens, human / Cell Type: fibroblast / Tissue: skin / Disease: normal General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits View Restrictions Organism Homo sapiens, human Tissue skin Cell Type fibroblast Product Format frozen Morphology fibroblast Culture Properties adherent Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease normal Age 43 years Gender male Ethnicity Caucasian Applications This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64) was derived. Thus, the two lines provide normal and melanoma tumor counterparts for comparative in vitro studies. Storage Conditions liquid nitrogen vapor phase Karyotype This is a normal diploid human cell line with 46, XY karyotype and the modal chromosome number of 46 occurring in 78% of cells. No marker chromosomes were detected. Both X and Y chromosomes were single-copied and normal in morphology. Derivation This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64) was derived. Clinical Data 43 years adult Caucasian male Antigen Expression HLA A2, Aw30, B13, B40(+/-), DRw7 Genes Expressed HLA A2, Aw30, B13, B40(+/-), DRw7 Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%. Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Interval: Subculture every 6 to 8 days Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week Cryopreservation Culture medium, 95%; DMSO, 5% Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C STR Profile Amelogenin: X,YCSF1PO: 10,12D13S317: 8,13D16S539: 9,12D5S818: 11,13D7S820: 9,12THO1: 7,8TPOX: 8,9vWA: 15,16 Isoenzymes AK-1, 1ES-D, 1G6PD, BGLO-I, 2Me-2, 1PGM1, 1PGM3, 1 Name of Depositor J Fogh References Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS Restrictions The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact email: firstname.lastname@example.org References Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.