CCD-1138Sk (ATCC® CRL-2707)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 43 years
Gender male
Ethnicity Caucasian
Applications
The cell line was derived from normal skin taken from the abdomen.
Storage Conditions liquid nitrogen vapor phase
Derivation
The cell line was derived from normal skin taken from the abdomen.
Clinical Data
male
Caucasian
43 years
Comments
The cell line was derived from normal skin taken from the abdomen.
Cells senesce after approximately 25 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor P.Wood, ATCC
References

Cells from the current passage (2) have experienced approximately 4.5 population doublings beyond the biopsy material. The cells are capable of at least 13 additional population doublings before the onset of senescence.

skin taken from the abdomen

Basic Documentation
References

Cells from the current passage (2) have experienced approximately 4.5 population doublings beyond the biopsy material. The cells are capable of at least 13 additional population doublings before the onset of senescence.

skin taken from the abdomen