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Dubca (ATCC® CRL-2276™)

Organism: Camelus dromedarius, camel  /  Cell Type: SV40 transformed  /  Tissue: skin  /  Disease: Autopsy

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Organism Camelus dromedarius, camel
Tissue skin
Cell Type SV40 transformed
Product Format frozen
Morphology fibroblast
Culture Properties monlayer
Biosafety Level 2   [Cells contain papovavirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Autopsy
Age 2 months gestation
Gender female
Applications
SV40 large T-antigen is detected by Western blotting.
The cell line is susceptible to infection with viruses of several different families.
The cells may be used for virus diagnosis and vaccine production.
Derivation
The cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE.
Clinical Data
female
Virus Resistance
parapox virus; canine adenovirus type 1 (CAV); Aleutian disease virus (ADV); influenza virus (A equi 1); ECBO virus, equine arteritis virus (EAV)
Comments
The cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE.
Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar.
SV40 large T-antigen is detected by Western blotting.
The cell line is susceptible to infection with viruses of several different families.
Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages.
While some isolates of bovine viral diarrhea virus (BVDV) and border disease virus (BDV) could not be grown in Dubca cells other isolates could be propagated to a limited extent (only a few cells expressed viral antigen).
The cells may be used for virus diagnosis and vaccine production.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor OR Kaaden
Passage History
Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar.
Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages.
References

Klopries M, et al. Characterization of the camel skin cell line Dubca. Br. Vet. J. 151: 555-565, 1995. PubMed: 8556315

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Klopries M, et al. Characterization of the camel skin cell line Dubca. Br. Vet. J. 151: 555-565, 1995. PubMed: 8556315