A375.S2 (ATCC® CRL-1872)

Organism: Homo sapiens, human  /  Cell Type: Melanoma  /  Tissue: skin  /  Disease: malignant melanoma

Organism Homo sapiens, human
Tissue
skin
Cell Type Melanoma
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease malignant melanoma
Age 54 years
Gender female
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was cloned by limiting dilution from the A375 melanoma cell line (see ATCC CRL-1619).
Clinical Data
female
Tumorigenic Yes
Effects
Yes, in immunosuppressed mice
Comments
These cells will eventually die and lyse when cultured in medium containing interleukin-1 (interleukin 1, IL-1) and therefore can be used in a reliable and sensitive growth inhibition assay for IL-1.
Sensitivity to IL-1 is maximal when the cells are derived from subconfluent cultures in logarithmic growth.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution and let the culture sit at room temperature (or 37°C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17
Basic Documentation
References

Onozaki K, et al. Human interleukin-1 is a cytocidal factor for several tumor cell lines. J. Immunol. 135: 3962-3968, 1985. PubMed: 2415593

Lachman LB, et al. Natural and recombinant human interleukin-1beta is cytotoxic for human melanoma cells. J. Immunol. 136: 3098-3102, 1986. PubMed: 3485680

Nakai S, et al. A simple, sensitive bioassay for the detection of interleukin-1 using human melanoma A375 cell line. Biochem. Biophys. Res. Commun. 154: 1189-1196, 1988. PubMed: 3261584

Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758