C32TG [C32-r16TG] (ATCC® CRL-1579)

Organism: Homo sapiens, human  /  Tissue: skin  /  Disease: amelanotic melanoma

Organism Homo sapiens, human
Tissue skin
Product Format frozen
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease amelanotic melanoma
Age 53 adult
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived from C32 (ATCC CRL-1585) by selection for 6-thioguanine resistance.
Clinical Data
male
Caucasian
53 adult
Tumorigenic Yes
Effects
Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments

The cells are resistant to 6-thioguanine and sensitive to HAT medium.

They are deficient in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-).

Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, 90%; fetal bovine serum, 10%; 0.050 mM 6-thioguanine
Subculturing
Remove medium, add fresh 0.25% trypsin solution several minutes until cells detach. Add fresh medium and centrifuge. Resuspend in fresh medium and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation

Freeze medium: Culture medium, 95%; DMSO, 5%.

STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 12
D7S820: 9,11
THO1: 9.3
TPOX: 9
vWA: 14,19
Name of Depositor TR Chen
References

Chen TR. Chromosome changes in 6-TG-resistant mutant strains derived from a karyotypically stable human line, C32. Cytogenet. Cell Genet. 35: 181-189, 1983. PubMed: 6861522

Chen TR. Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277-284, 1978. PubMed: 277716

Chen TR, Shaw MW. Stable chromosome changes in human malignant melanoma. Cancer Res. 33: 2042-2047, 1973. PubMed: 4725368

Basic Documentation
References

Chen TR. Chromosome changes in 6-TG-resistant mutant strains derived from a karyotypically stable human line, C32. Cytogenet. Cell Genet. 35: 181-189, 1983. PubMed: 6861522

Chen TR. Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277-284, 1978. PubMed: 277716

Chen TR, Shaw MW. Stable chromosome changes in human malignant melanoma. Cancer Res. 33: 2042-2047, 1973. PubMed: 4725368