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Da Hol (ATCC^® CRL-1379^™)

Organism: Homo sapiens, human /

Da Hol ATCC^® CRL-1379^™

frozen

For-Profit: $551.00 Non-Profit: $459.17

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Permits and Restrictions	View Permits
Organism	Homo sapiens, human
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1
Age	8 years
Gender	female

Clinical Data	The tissue donor suffered from a variant form of Ehlers-Danlos syndrome. female
Comments	The tissue donor suffered from a variant form of Ehlers-Danlos syndrome.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS