Ro Dow (ATCC® CRL-1337)

Organism: Homo sapiens, human  /  Tissue: skin  /  Disease: Ehlers-Danlos syndrome, type II

Organism Homo sapiens, human
Tissue skin
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Ehlers-Danlos syndrome, type II
Age 65 years
Gender female
Clinical Data
The tissue donor suffered from type II Ehlers-Danlos syndrome.
The tissue donor suffered from type II Ehlers-Danlos syndrome.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 to 14 days.