Indian Muntjac (ATCC® CCL-157)

Organism: muntjac  / 

Organism muntjac
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)
Age adult
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 7; range = 7 to 14.
Stable karyotype in male diploid stemline number. Chromosome breaks noted in 6% of the cells examined.
Clinical Data
male
Virus Susceptibility Herpes simplex virus
Vaccinia virus
Vesicular stomatitis virus
Human poliovirus 1
Comments
Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).
Complete Growth Medium Ham's F10 medium, 80%; fetal bovine serum, 20%
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor DH Wunsten
Year of Origin November, 1969
References

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Wurster DH, Benirschke K. Indian muntjac, Muntiacus muntjak: a deer with a low diploid chromosome number. Science 168: 1364-1366, 1970. PubMed: 5444269

Basic Documentation
References

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Wurster DH, Benirschke K. Indian muntjac, Muntiacus muntjak: a deer with a low diploid chromosome number. Science 168: 1364-1366, 1970. PubMed: 5444269