AtT-20 (ATCC® CCL-89)

Organism: Mus musculus, mouse  /  Tissue: pituitary  /  Disease: tumor

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Tissue pituitary
Product Format frozen
Morphology small rounded cells
Biosafety Level 1
Disease tumor
Strain LAF1
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation Clone AtT-20, an ACTH secreting cell line, was cloned in October, 1966, by G. Sato and associates from earlier cultures established after alternate passage of mouse pituitary tumor cells as tumors in animals and in cell culture. (The cells were cultured in Ham's F10 medium, 82.5%; horse serum, 15%; FBS, 2.5%). The mouse pituitary tumor was originally established in LAF1 mice by J. Furth, et al. This clone has now been carried in culture without alternate animal passage.
Genes Expressed
adrenocorticotropic hormone (ACTH)
Virus Susceptibility Vesicular stomatitis virus
Human poliovirus 1
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Subculturing
Allow clusters to settle and remove all but 5 to 10 mL of medium. Gently transfer the clusters to new flasks and add fresh medium. Proliferation is enhanced by incubation on a shaking platform.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Cryopreservation
Freeze medium: culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Growth Conditions: The cells do not attach but aggregate to form clusters; the clusters are viable.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor G Sato
Passage History This clone has now been carried in culture without alternate animal passage.
Year of Origin October, 1966
References

Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682

Furth J, et al. ACTH secreting transplantable pituitary tumors. Proc. Soc. Exp. Biol. Med. 84: 253-254, 1953. PubMed: 13121002

Tabernero C, et al. Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1. J. Virol. 71: 95-101, 1997. PubMed: 8985327

Gamby C, et al. Growth-associated protein-43 (GAP-43) facilitates peptide hormone secretion in mouse anterior pituitary AtT-20 cells. J. Biol. Chem. 271: 10023-10028, 1996. PubMed: 8626556

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682

Furth J, et al. ACTH secreting transplantable pituitary tumors. Proc. Soc. Exp. Biol. Med. 84: 253-254, 1953. PubMed: 13121002

Tabernero C, et al. Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1. J. Virol. 71: 95-101, 1997. PubMed: 8985327

Gamby C, et al. Growth-associated protein-43 (GAP-43) facilitates peptide hormone secretion in mouse anterior pituitary AtT-20 cells. J. Biol. Chem. 271: 10023-10028, 1996. PubMed: 8626556