SVR bag4 (ATCC® CRL-2387)

Organism: Mus musculus, mouse  /  Cell Type: endothelialSV40 transformed  / 

Organism Mus musculus, mouse
Cell Type endothelialSV40 transformed
Product Format frozen
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
SVR bag4 is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280) by infecting SVR cells with a retrovirus encoding beta-galactosidase and puromycin resistance.
SVR bag4 can be used as a wild-type control for SVR A221a (ATCC CRL-2386).
It can be used to determine if drugs regulate the MAPKK pathway.
Derivation
SVR bag4 is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280) by infecting SVR cells with a retrovirus encoding beta-galactosidase and puromycin resistance.
Comments
SVR bag4 is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280) by infecting SVR cells with a retrovirus encoding beta-galactosidase and puromycin resistance.
SVR is a hygromycin resistant mouse endothelial cell line containing a temperature sensitive SV40 large T-antigen and H-RAS oncogene.
SVR bag4 can be used as a wild-type control for SVR A221a (ATCC CRL-2386). It can be used to determine if drugs regulate the MAPKK pathway.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 1.0 g/L glucose, 95%; fetal bovine serum, 5%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor JL Arbiser
Deposited As mouse
References

LaMontagne KR Jr., et al. Inhibition of MAP kinase kinase causes morphological reversion and dissociation between soft agar growth and in vivo tumorigenesis in angiosarcoma cells. Am. J. Pathol. 157: 1937-1945, 2000. PubMed: 11106566

Basic Documentation
References

LaMontagne KR Jr., et al. Inhibition of MAP kinase kinase causes morphological reversion and dissociation between soft agar growth and in vivo tumorigenesis in angiosarcoma cells. Am. J. Pathol. 157: 1937-1945, 2000. PubMed: 11106566