TGP47 (ATCC® CRL-2141)

Organism: Mus musculus, transgenic, mouse, transgenic  /  Cell Type:: Epithelial  /  Tissue: pancreas  /  Disease: pancreatic acinar cell tumor; carcinoma

Permits and Restrictions

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Organism Mus musculus, transgenic, mouse, transgenic
Tissue pancreas
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 CELLS CONTAIN PAPOVAVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease pancreatic acinar cell tumor; carcinoma
Age 26 weeks
Gender male
Strain Tg(E1a-1-SV40E)Bri18
Applications
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
The cells do not secrete amylase or lipase.
Histochemical staining of the current stock of TGP47 was negative for somatostatin and positive for trypsin.
Derivation
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
Clinical Data
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
male
Comments
TGP47 is an epithelial like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
TGP47 cells appear somewhat duct like in culture, but contain rare somatostatin positive cells.
The cells do not secrete amylase or lipase.
Histochemical staining of the current stock of TGP47 was negative for somatostatin and positive for trypsin.
Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture.
Complete Growth Medium A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor OS Pettengill, D Longnecker
References

Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.

References

Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904