DSL-6A/C1 (ATCC® CRL-2132)

Organism: Rattus norvegicus, rat  /  Disease: pancreatic carcinoma, azaserine induced

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Organism Rattus norvegicus, rat
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease pancreatic carcinoma, azaserine induced
Gender male
Strain Lewis
Applications
DSL-6A/C1 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma.
The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat (DSL-101-79) that was given azaserine intraperitoneally.
The cultured DSL-6A/C1 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture.
The DSL-6A/C1 cell line expresses the ductal marker cystic fibrosis transmembrane regulator (CFTR).
Derivation
DSL-6A/C1 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma.
The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat (DSL-101-79) that was given azaserine intraperitoneally.
The cultured DSL-6A/C1 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture.
Clinical Data
The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat (DSL-101-79) that was given azaserine intraperitoneally.
male
Tumorigenic Yes
Effects
Yes, in Lewis rats the cells produce solid tumors composed of ductlike structures surrounded by dense fibrous tissue
Comments
DSL-6A/C1 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma.
The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat (DSL-101-79) that was given azaserine intraperitoneally.
The cultured DSL-6A/C1 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture.
The cell line also lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and regrafting.
The DSL-6A/C1 cell line expresses the ductal marker cystic fibrosis transmembrane regulator (CFTR).
Complete Growth Medium Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37C.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor OS Pettengill, D Longnecker
Year of Origin 1986
References

Durfee T, et al. Derivation of ductlike cell lines from a transplantable acinar cell carcinoma of the rat pancreas. Am. J. Pathol. 143: 292-303, 1993. PubMed: 8391218

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.

References

Durfee T, et al. Derivation of ductlike cell lines from a transplantable acinar cell carcinoma of the rat pancreas. Am. J. Pathol. 143: 292-303, 1993. PubMed: 8391218