DUKX B1 [5A HS MYC] (ATCC® CRL-9010)

Organism: Cricetulus griseus, hamster, Chinese  / 

Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology epithelial
Culture Properties adherent, adherent
Biosafety Level 1
Gender female
Storage Conditions liquid nitrogen vapor phase
Clinical Data
Genes Expressed
over produces mouse c-myc protein when heated
Cellular Products
over produces mouse c-myc protein when heated
The cells are reported to contain DNA sequences from mouse, drosophila, adenovirus and E. coli.
Complete Growth Medium Alpha modified Eagle's minimum essential medium with 0.32 mM methotrexate, 90%; fetal bovine serum, 10%
Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Freeze medium: culture medium ,95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor R Kingston