5A HS MYC (ATCC® CRL-9010)

Organism: Cricetulus griseus, hamster, Chinese  /  Tissue: ovary  / 

Organism Cricetulus griseus, hamster, Chinese
Tissue ovary
Product Format frozen
Morphology epithelial
Culture Properties adherent, adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender female
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation

Derived from the parental cell line - CHO-DUKKX BII

Clinical Data
female
Genes Expressed
over produces mouse c-myc protein when heated
Cellular Products
over produces mouse c-myc protein when heated
Comments
The cells are reported to contain DNA sequences from mouse, drosophila, adenovirus and E. coli.
Complete Growth Medium Alpha modified Eagle's minimum essential medium with 0.32 mM methotrexate, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium ,95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor R Kingston
U.S. Patent Number European Patent No.: EP0232845 A2
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References

Wurm FM, et al. Inducible overproduction of the mouse c-myc protein in mammalian cells. Proc Natl Acad Sci 83(15): 5414-5418, 1986. PubMed: 3526329

Basic Documentation
References

Wurm FM, et al. Inducible overproduction of the mouse c-myc protein in mammalian cells. Proc Natl Acad Sci 83(15): 5414-5418, 1986. PubMed: 3526329