CHO-ICAM-1 (ATCC® CRL-2093)

Organism: Cricetulus griseus, hamster, Chinese  / 

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Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology epithelial
Culture Properties adherent, adherent
Biosafety Level 1
Gender female
Applications
The cells can be used in adherence assays as a target cell for malaria infected erythrocytes.
Functional expression of the transfected receptor molecules on CHO-ICAM-1 was confirmed using parasites with different cytoadherence phenotypes and cytoadherence inhibition experiments with a panel of anti ICAM-1 antibodies.
The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies.
Storage Conditions liqid nitrogen vapor phase
Derivation
The CHO-ICAM-1 cell line was constructed at DNAX Research Institute, Palo Alto, California by transfecting Chinese hamster ovary (CHO) cells with linearized cDNA of human ICAM-1 in pCDM8 by electroporation in a 10 fold molar excess over pBJ1-Neo.
The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies.
Clinical Data
The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies.
female
Comments
The CHO-ICAM-1 cell line was constructed at DNAX Research Institute, Palo Alto, California by transfecting Chinese hamster ovary (CHO) cells with linearized cDNA of human ICAM-1 in pCDM8 by electroporation in a 10 fold molar excess over pBJ1-Neo.
Stable transfectants were then selected by growth in culture medium containing G418.
CHO-ICAM-1 cells express high levels of human ICAM-1 (a ligand for erythrocytes infected with the human malaria parasite, Plasmodium falciparum).
The cells can be used in adherence assays as a target cell for malaria infected erythrocytes.
Functional expression of the transfected receptor molecules on CHO-ICAM-1 was confirmed using parasites with different cytoadherence phenotypes and cytoadherence inhibition experiments with a panel of anti ICAM-1 antibodies.
The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies.
A culture submitted to the ATCC in August 1993 was found to be contaminated with mycoplasma, and the line was cured by a 21 day treatment with BM Cycline.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liqid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor BL Pasloske
References

Hasler T, et al. An improved microassay for Plasmodium falciparum cytoadherence using stable transformants of Chinese hamster ovary cells expressing CD36 or intercellular adhesion molecule-1. Am. J. Trop. Med. Hyg. 48: 332-347, 1993. PubMed: 7682381

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hasler T, et al. An improved microassay for Plasmodium falciparum cytoadherence using stable transformants of Chinese hamster ovary cells expressing CD36 or intercellular adhesion molecule-1. Am. J. Trop. Med. Hyg. 48: 332-347, 1993. PubMed: 7682381