Complete Growth Medium
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Ham's F12 medium with 0.05 to 0.1 mg/ml G418, 90%; fetal bovine serum, 10%
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Subculturing
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Protocol: Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature (or incubate at 37C) until the cells detach (usually 5 to 10 minutes). Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: At the time of subcultivation
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Cryopreservation
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Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
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