AA8 (ATCC® CRL-1859)

Organism: Cricetulus griseus, hamster, Chinese  / 

Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology epithelial-like
Culture Properties monolayer and suspension
Biosafety Level 1
Gender female
Applications
The line is useful for gene mutation assays and is the parent line for a series of repair deficient mutants (see ATCC CRL-1860, ATCC CRL-1861, ATCC CRL-1862, ATCC CRL-1865, ATCC CRL-1866 and ATCC CRL-1867).
Karyotype chromosome number = 21
Derivation
This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).
Clinical Data
female
Comments
This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).
The AA8 line has 21 chromosomes and is heterozygous at the aprt locus.
The line is useful for gene mutation assays and is the parent line for a series of repair deficient mutants (see ATCC CRL-1860, ATCC CRL-1861, ATCC CRL-1862, ATCC CRL-1865, ATCC CRL-1866 and ATCC CRL-1867).
Complete Growth Medium Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
Medium Renewal: 2 to 3 times per week
To subculture attached cells, remove the medium and rinse the cells with fresh 0.25% trypsin, 0.03% EDTA.
Let the culture sit at 37C until the cells detach (about 5 minutes).
Add fresh medium, aspirate and transfer to new flasks. The suspended cells are viable and can be used to start new cultures.
Population Doubling Time 12 hrs
Name of Depositor LH Thompson
References

Thompson LH, et al. Validation of conditions for efficient detection of HPRT and APRT mutations in suspension-cultured Chinese hamster ovary cells. Mutat. Res. 74: 21-36, 1980. PubMed: 7360155

Bessho T, et al. Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5" to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand. Mol. Cell. Biol. 17: 6822-6830, 1997. PubMed: 9372913

Reardon JT, et al. Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAKand TFIIH. Proc. Natl. Acad. Sci. USA 93: 6482-6487, 1996. PubMed: 8692841

Basic Documentation
References

Thompson LH, et al. Validation of conditions for efficient detection of HPRT and APRT mutations in suspension-cultured Chinese hamster ovary cells. Mutat. Res. 74: 21-36, 1980. PubMed: 7360155

Bessho T, et al. Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5" to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand. Mol. Cell. Biol. 17: 6822-6830, 1997. PubMed: 9372913

Reardon JT, et al. Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAKand TFIIH. Proc. Natl. Acad. Sci. USA 93: 6482-6487, 1996. PubMed: 8692841