B13-24 (ATCC® CRL-11397)

Organism: Cricetulus griseus, hamster, Chinese  / 

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Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Culture Properties adherent
Biosafety Level 2 CELLS CONTAIN SV-40-CMV VIRAL DNA SEQUENCES
Gender female
Applications
The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).
The cell line is capable of producing about 100 micrograms/liter of the humanized immunoglobulin at the rate of 25 pg/cell/day.
Derivation
The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).
Clinical Data
female
Comments
This line is Neomycin resistant.
The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).
The plasmids contain both cytomegalovirus (CMV) and SV40 viral sequences.
The 23F2G antibody is reactive with the beta chain (CD18) of human leukocyte integrins.
The cell line is capable of producing about 100 micrograms/liter of the humanized immunoglobulin at the rate of 25 pg/cell/day.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG4
Name of Depositor ICOS Corporation
References

Rose LM. Murine and humanizer 23F2G antibodies and cell lines expressing said antibodies. US Patent 5,854,070 dated Dec 29 1998

Bowen JD, et al. Phase I study of a humanized anti-CD11/CD18 monoclonal antibody in multiple sclerosis. Clin. Pharmacol. Ther. 64: 339-346, 1998. PubMed: 9757158

Yenari MA, et al. Hu23F2G, an antibody recognizing the leukocyte CD11/CD18 integrin, reduces injury in a rabbit model of transient focal cerebral ischemia. Exp. Neurol. 153: 223-233, 1998. PubMed: 9784282

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rose LM. Murine and humanizer 23F2G antibodies and cell lines expressing said antibodies. US Patent 5,854,070 dated Dec 29 1998

Bowen JD, et al. Phase I study of a humanized anti-CD11/CD18 monoclonal antibody in multiple sclerosis. Clin. Pharmacol. Ther. 64: 339-346, 1998. PubMed: 9757158

Yenari MA, et al. Hu23F2G, an antibody recognizing the leukocyte CD11/CD18 integrin, reduces injury in a rabbit model of transient focal cerebral ischemia. Exp. Neurol. 153: 223-233, 1998. PubMed: 9784282