BEND (ATCC® CRL-2398)

Organism: Bos taurus, cow  /  Tissue: uterus; endometrium  / 

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Organism Bos taurus, cow
Tissue uterus; endometrium
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain bovine viral diarrhea virus (BVDV)]
Age adult; day 14 of estrous cycle
Gender female
Storage Conditions liquid nitrogen vapor phase
Derivation
The BEND cell line was derived from the uterine endometrium of a normal female cow on day 14 of the estrous cycle in 1997 in Laramie Wyoming, United States.
Comments
The cells produce the pregnancy associated protein called bovine ubiquitin cross-reactive protein (UCRP) and alpha chemokines in response to the cytokine interferon-tau.

BEND was submitted to the American Type Culture Collection in January, 1998 at passage 6.

Tests for bovine viral diarrhea virus (BVDV) at the ATCC have indicated that BEND cells are positive for BVDV by immunofluorescence.
Complete Growth Medium 1:1 mixture of Ham's F12 and Eagle's Minimal Essential medium with Earle's BSS (D-valine modification) with 1.5 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.034 g/L D-valine, 10% heat-inactivated fetal bovine serum and 10% heat-inactivated horse serum
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:4 to 1:6
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor TR Hansen, KJ Austin, GA Johnson
Passage History
BEND was submitted to the American Type Culture Collection in January, 1998 at passage 6.
Year of Origin 1997
References

Staggs KL, et al. Complex induction of bovine uterine proteins by interferon-tau. Biol. Reprod. 59: 293-297, 1998. PubMed: 9687298

Hansen TR, et al. Transient ubiquitin cross-reactive protein gene expression in the bovine endometrium. Endocrinology 138: 5079-5082, 1997. PubMed: 9348245

Austin KJ, et al. Pregnancy-specific protein B induces release of an alpha chemokine in bovine endometrium. Endocrinology 140: 542-545, 1999. PubMed: 9886868

Johnson GA, et al. Endometrial ISG17 mRNA and a related mRNA are induced by interferon-tau and localized to glandular epithelial and stromal cells from pregnant cows. Endocrine 10: 243-252, 1999. PubMed: 10484288

Perry DJ, et al. Cloning of interferon-stimulated gene 17: the promoter and nuclear proteins that regulate transcription. Mol. Endocrinol. 13: 1197-1206, 1999. PubMed: 10406469

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation
References

Staggs KL, et al. Complex induction of bovine uterine proteins by interferon-tau. Biol. Reprod. 59: 293-297, 1998. PubMed: 9687298

Hansen TR, et al. Transient ubiquitin cross-reactive protein gene expression in the bovine endometrium. Endocrinology 138: 5079-5082, 1997. PubMed: 9348245

Austin KJ, et al. Pregnancy-specific protein B induces release of an alpha chemokine in bovine endometrium. Endocrinology 140: 542-545, 1999. PubMed: 9886868

Johnson GA, et al. Endometrial ISG17 mRNA and a related mRNA are induced by interferon-tau and localized to glandular epithelial and stromal cells from pregnant cows. Endocrine 10: 243-252, 1999. PubMed: 10484288

Perry DJ, et al. Cloning of interferon-stimulated gene 17: the promoter and nuclear proteins that regulate transcription. Mol. Endocrinol. 13: 1197-1206, 1999. PubMed: 10406469

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm