6-23 (Clone 6) (ATCC® CRL-1607)

Organism: Rattus norvegicus, rat  /  Cell Type: C cell  /  Tissue: thyroid  /  Disease: medullary thyroid carcinoma

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Organism Rattus norvegicus, rat
Tissue thyroid
Cell Type C cell
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease medullary thyroid carcinoma
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
neurotensin; calcitonin
Comments
Secretion of neurotensin and calcitonin is stimulated by calcium, KCl, glucagon and catecholamines.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; horse serum, 15%
Subculturing
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 minute, remove trypsin EDTA, add 0.3 mL of 0.25% trypsin and let the culture sit at 37°C for 6 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 to 12 days.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: 3 times per week
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Name of Depositor R Gagel, FN Zeytinoglu
References

Zeytinoglu FN, et al. Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. Morphological studies of the tumor and cells in culture. Endocrinology 107: 509-515, 1980. PubMed: 6248326

Zeytinoglu FN, et al. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc. Natl. Acad. Sci. USA 77: 3741-3745, 1980. PubMed: 6932046

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Zeytinoglu FN, et al. Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. Morphological studies of the tumor and cells in culture. Endocrinology 107: 509-515, 1980. PubMed: 6248326

Zeytinoglu FN, et al. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc. Natl. Acad. Sci. USA 77: 3741-3745, 1980. PubMed: 6932046