FRTL (ATCC® CRL-1468)

Organism: Rattus norvegicus, rat  /  Tissue: thyroid  /  Disease: normal

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Organism Rattus norvegicus, rat
Tissue
thyroid
Product Format frozen
Morphology epithelial
Culture Properties adherent, patchy
Biosafety Level 1
Disease normal
Age 6 weeks
Strain Fischer
Storage Conditions liquid nitrogen vapor phase
Karyotype diploid
Images
Derivation Epithelial cells from rat thyroids by HG Coon.
Genes Expressed
thyroglobulin
Tumorigenic No
Effects
No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
The cells accumulate iodide intracellularly.
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with: 10 mU/ml TSH, 0.01 mg/ml insulin 10 nM hydrocortisone 0.005 mg/ml transferrin 10 ng/ml somatostatin 10 ng/ml glycyl-L-histidyl-L-lysine acetate 0.5 % bovine calf serum (All are obtainable from Sigma, Collaborative Research and/or Calbiochem.)
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

There is an absolute requirement for 0.1 to 0.5% calf serum in addition to insulin, hydrocortisone, thyrotropin, transferrin, somatostatin and glycyl-1-histidyl-lysine acetate.

The cells tend to grow one above another, forming three dimensional structures rather than expanding into a monolayer.

Subculture before “domes” form, flasks never become confluent.

  1. Remove and discard culture medium.
  2. Add 2.0 to 3.0 mL of CTC solution ( Collagenase, 20 U/mL; Trypsin, 0.075%; dialyzed heat-inactivated Chicken serum, 2%) to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  4. To remove trypsin-collagenase solution, transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
  5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
  6. Place culture vessels in incubators at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor HG Coon
References

Ambesi-Impiombato FS, et al. Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc. Natl. Acad. Sci. USA 77: 3455-3459, 1980. PubMed: 6106191

Bidey SP, et al. Evaluation of the rat thyroid cell strain FRTL-5 as an in-vitro bioassay system for thyrotrophin. J. Endocrinol. 101: 269-276, 1984. PubMed: 6327870

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Ambesi-Impiombato FS, et al. Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc. Natl. Acad. Sci. USA 77: 3455-3459, 1980. PubMed: 6106191

Bidey SP, et al. Evaluation of the rat thyroid cell strain FRTL-5 as an in-vitro bioassay system for thyrotrophin. J. Endocrinol. 101: 269-276, 1984. PubMed: 6327870