sNF02.2 (ATCC® CRL-2885)

Organism: Homo sapiens, human  /  Cell Type: Schwann Cell  /  Tissue: derived from metastatic site: lung  /  Disease: neurofibrmatosis type 1 (NF1)

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Organism Homo sapiens, human
Tissue derived from metastatic site: lung
Cell Type Schwann Cell
Product Format frozen
Morphology Schwann cell-like
Culture Properties adherent
Biosafety Level 1
Disease neurofibrmatosis type 1 (NF1)
Age 35 year old
Gender male
Storage Conditions Liquid nitrogen vapor phase
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Derivation
The sNF02.2 was derived from a lung metastasis diagnosed by histopathology as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. Western immunoblotting showed apparently full-length neurofibromin protein.
Clinical Data
35 years
male
Comments
Western immunoblotting showed apparently full-length neurofibromin protein.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2. flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 X 103 to 8 X 103 viable cells/cm2 is recommended.
  7. Incubate cultures at 37°C. Subculture when cell concentration is between 3 X 104 and 4 X 104 cells/cm2.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 twice weekly is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Culture medium,90%; DMSO,10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11,12
D5S818: 12
D7S820: 10,11
THO1: 7,9
TPOX: 8,12
vWA: 16
Population Doubling Time about 26 hours
Name of Depositor DF Muir, MR Wallace
Passage History
­The cells were established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75. ­
Year of Origin 2002
References

Fieber LA, et al. Delayed rectifier K currents in NF1 Schwann cells. Pharmacological block inhibits proliferation. Neurobiol. Dis. 13: 136-146, 2003. PubMed: 12828937

Li Y, et al. Notch and Schwann cell transformation. Oncogene 23: 1146-1152, 2004. PubMed: 14762442

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Fieber LA, et al. Delayed rectifier K currents in NF1 Schwann cells. Pharmacological block inhibits proliferation. Neurobiol. Dis. 13: 136-146, 2003. PubMed: 12828937

Li Y, et al. Notch and Schwann cell transformation. Oncogene 23: 1146-1152, 2004. PubMed: 14762442