Yes, the cells form colonies in soft agar
RefRidley AJ, et al. Ras-mediated cell cycle arrest is altered by nuclear oncogenes to induce Schwann cell transformation. EMBO J. 7: 1635-1645, 1988. PubMed: 3049071
SV40-tsA58 large T antigen [PubMed:3049071]
Myelin-associated glycoprotein (Mag); Protein and mRNA, negative [PubMed: 12210843]
Myelin basic protein (Mbp); Protein and mRNA, negative [PubMed: 12210843]
Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); Protein and mRNA, negative [PubMed: 12210843]
peripheral myelin protein 22 (Pmp22); protein, negative; mRNA, positive; [PubMed: 12210843]
S100 calcium-binding protein, beta (neural) (S100b) protein, positive [PubMed: 12210843]Receptors:
Nerve growth factor receptor (Ngfr) Protein and mRNA, negative
Primary cells were infected with the recombinant retrovirus LJ-tsSVLT containing the temperature sensitive tsA58 large SV40 T oncogene and a gene encoding G418 resistance. Clones were selected in the presence of 0.4 mg/ml G418 and super-infected with Zipras 6 containing v-Ha-ras at the permissive temperature of 33C [PubMed:3049071]. The cells proliferate at both the permissive temperature of 33C and the restrictive temperature of 39.5C. However, the growth rate is increased and growth factor requirements are reduced at the permissive temperature [PubMed: 3049071]. At the restrictive temperature, expression of large T antigen was not detected [PubMed: 3049071]. Expression of p21-v-Ha-ras was not detected [PubMed: 3049071].
A culture submitted to the ATCC in December of 2002 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.