4/4 R.M.-4 (ATCC® CCL-216)

Organism: Rattus norvegicus, rat  / 

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Organism Rattus norvegicus, rat
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 2 to 3 months
Gender female
Clinical Data
female
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Remove medium, rinse cell sheet once with fresh 0.25% trypsin and let the culture sit for 10 to 12 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor JF Aronson
References

Aronson JF, Cristofalo VJ. Growth of epithelial cells from rat pleura. In Vitro 15: 214, 1979.

Aronson JF, Cristofalo VJ. Culture of epithelial cells from the rat pleura. In Vitro 17: 61-70, 1981. PubMed: 7216240

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Aronson JF, Cristofalo VJ. Growth of epithelial cells from rat pleura. In Vitro 15: 214, 1979.

Aronson JF, Cristofalo VJ. Culture of epithelial cells from the rat pleura. In Vitro 17: 61-70, 1981. PubMed: 7216240