BeWo (ATCC® CCL-98)

Tissue: placenta  /  Disease: choriocarcinoma

Tissue
placenta
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease choriocarcinoma
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 86; range = 71 to 178.
Stemline number is hypotetraploid. Karyotype relatively stable within stemline number.
Clinical Data
male
Genes Expressed
hormones; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol. The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
hormones; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol; keratin
Virus Susceptibility Human poliovirus 3
Vesicular stomatitis virus
Comments
The cells are positive for keratin by immunoperoxidase staining.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 3 to 4 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 13,14
D5S818: 10,11
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 16
Isoenzymes
G6PD, B
Name of Depositor RA Pattillo
References

Pattillo RA, et al. Human hormone production in vitro. Science 159: 1467-1469, 1968. PubMed: 5753554

Pattillo RA, et al. Control mechanisms for gonadotrophic hormone production in vitro. In Vitro 6: 205-214, 1970. PubMed: 5535575

Pattillo RA, et al. Estrogen production by trophoblastic tumors in tissue culture. J. Clin. Endocrinol. Metab. 34: 59-61, 1972. PubMed: 4332667

Pattillo RA, Gey GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 28: 1231-1236, 1968. PubMed: 4299001

Hertz R. Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc. Soc. Exp. Biol. Med. 102: 77-81, 1959. PubMed: 14401422

Heckert LL, et al. The cAMP response elements of the alpha subunit gene bind similar proteins in trophoblasts and gonadotropes but have distinct functional sequence requirements. J. Biol. Chem. 49: 31650-31656, 1996. PubMed: 8940185

Basic Documentation
References

Pattillo RA, et al. Human hormone production in vitro. Science 159: 1467-1469, 1968. PubMed: 5753554

Pattillo RA, et al. Control mechanisms for gonadotrophic hormone production in vitro. In Vitro 6: 205-214, 1970. PubMed: 5535575

Pattillo RA, et al. Estrogen production by trophoblastic tumors in tissue culture. J. Clin. Endocrinol. Metab. 34: 59-61, 1972. PubMed: 4332667

Pattillo RA, Gey GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 28: 1231-1236, 1968. PubMed: 4299001

Hertz R. Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc. Soc. Exp. Biol. Med. 102: 77-81, 1959. PubMed: 14401422

Heckert LL, et al. The cAMP response elements of the alpha subunit gene bind similar proteins in trophoblasts and gonadotropes but have distinct functional sequence requirements. J. Biol. Chem. 49: 31650-31656, 1996. PubMed: 8940185