Fc3Tg (ATCC® CCL-176)

Organism: Felis catus, cat  / 

Organism Felis catus, cat
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age embryo
Gender female
Clinical Data
female
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.05% trypsin, 0.02% versene for 1 to 2 minutes, remove trypsin and allow the culture to sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor WA Nelson-Rees