Fc3Tg (ATCC® CCL-176)

Organism: Felis catus, cat  / 

Permits and Restrictions

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Organism Felis catus, cat
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age embryo
Gender female
Clinical Data
female
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.05% trypsin, 0.02% versene for 1 to 2 minutes, remove trypsin and allow the culture to sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor WA Nelson-Rees
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation