G1B (ATCC® CRL-2536)

Organism: Clarias batrachus, walking catfish  / 

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Organism Clarias batrachus, walking catfish
Product Format frozen
Morphology pleomorphic
Culture Properties adherent
Biosafety Level 1
Age adult
Applications
The G1B cell line was derived from normal gill tissue taken from a healthy adult walking catfish.
Derivation
The G1B cell line was derived from normal gill tissue taken from a healthy adult walking catfish.
Comments
The G1B cell line was derived from normal gill tissue taken from a healthy adult walking catfish.
The cells support the in vitro growth of Amyloodinium ocellatum, a dinoflagellate that infects the gills and skin of both marine and brackish water fishes.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 24 mM HEPES

Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin-0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 25.0°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. NOTE: Some batches of fetal bovine serum are toxic to these cells.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 25.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor EJ Noga
References

Noga EJ, Hartmann JX. Establishment of walking catfish (Clarias batrachus) cell lines and development of a channel catfish (Ictalurus punctatus) virus vaccine. Can. J. Fish. Aquat. Sci. 38: 925-930, 1981.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Noga EJ, Hartmann JX. Establishment of walking catfish (Clarias batrachus) cell lines and development of a channel catfish (Ictalurus punctatus) virus vaccine. Can. J. Fish. Aquat. Sci. 38: 925-930, 1981.