AB.9 (ATCC® CRL-2298)

Organism: Danio rerio, zebrafish  /  Cell Type: fibroblast  / 

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Organism Danio rerio, zebrafish
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Biosafety Level 1
Age adult
Strain AB
Applications
AB.9 is a fibroblast cell line derived from amputated caudal fins of an adult zebrafish, strain AB.
This strain of zebrafish is used for genetic mapping; it is the strain used by Leonard I. Zon at Harvard to generate the zebrafish YAC genomic library and by Peter Goodfellow at Cambridge, United Kingdom to generate the zebrafish radiation hybrid panel.
Storage Conditions liquid nitrogen vapor phase
Derivation
AB.9 is a fibroblast cell line derived from amputated caudal fins of an adult zebrafish, strain AB.
Comments
AB.9 is a fibroblast cell line derived from amputated caudal fins of an adult zebrafish, strain AB.
This strain of zebrafish is used for genetic mapping; it is the strain used by Leonard I. Zon at Harvard to generate the zebrafish YAC genomic library and by Peter Goodfellow at Cambridge, United Kingdom to generate the zebrafish radiation hybrid panel.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 28°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 28°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 28.0°C
Name of Depositor BH Paw, LI Zon
References

Paw BH, Zon LI. Primary fibroblast cell culture. Methods Cell Biol. 59: 39-43, 1999. PubMed: 9891354

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Paw BH, Zon LI. Primary fibroblast cell culture. Methods Cell Biol. 59: 39-43, 1999. PubMed: 9891354