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Ch 1 Es (NBL-8) (ATCC® CCL-73™)

Organism: Capra hircus, goat  / 

Permits and Restrictions

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Organism Capra hircus, goat
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age 2/3 term
Gender male
Applications
The cells are positive for bovine viral diarrhea virus (BVDV).
Clinical Data
male
Virus Resistance
poliovirus 3; coxsackievirus A9, B5; adenovirus 5
Comments
The cells are positive for bovine viral diarrhea virus (BVDV).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 1 to 2 times per week
Rinse cell sheet 2 twice with fresh ATV solution, remove ATV and allow the culture to stand at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor AJ Kniazeff, WA Nelson-Rees, NB Darby, R Owens
References

Nelson-Rees WA, et al. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436-450, 1967. PubMed: 5587551

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Nelson-Rees WA, et al. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436-450, 1967. PubMed: 5587551

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438