PA317 containing JR-gal (ATCC® CRL-9995)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  / 

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Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age embryo
Strain NIH/Swiss
Applications
This cell line was derived from PA317 (see ATCC CRL-9098) by introduction of a retrovirus vector (JR-gal).
The vector is derived from the JR vector by incorporation of the E. coli beta galactosidase gene (PstI to SalI fragment of pBAG gag - beta gal fusion protein) into the BamH1 site of JR.
Derivation
This cell line was derived from PA317 (see ATCC CRL-9098) by introduction of a retrovirus vector (JR-gal).
The vector is derived from the JR vector by incorporation of the E. coli beta galactosidase gene (PstI to SalI fragment of pBAG gag - beta gal fusion protein) into the BamH1 site of JR.
Comments
This cell line was derived from PA317 (see ATCC CRL-9098) by introduction of a retrovirus vector (JR-gal).
The vector is derived from the JR vector by incorporation of the E. coli beta galactosidase gene (PstI to SalI fragment of pBAG gag - beta gal fusion protein) into the BamH1 site of JR.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor Wisconsin Alumni Res. Fndn.
References

Gould MN, Wang B. Method for producing a recombinant mammal in vivo. US Patent 5,215,904 dated Jun 1 1993

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gould MN, Wang B. Method for producing a recombinant mammal in vivo. US Patent 5,215,904 dated Jun 1 1993