B2-1 (ATCC® CRL-8085)

Organism: Mus musculus, mouse  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age embryo
Strain BALB/c
Applications
B2-1 cells are thymidine kinase negative (TK-) and are resistant to IUdR.
Tumorigenic No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
B2-1 cells are thymidine kinase negative (TK-) and are resistant to IUdR.
Complete Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with: 0.05 mg/ml 5-iodo-2'-deoxyuridine 10% heat-inactivated fetal bovine serum
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Remove IUdR for one passage before using.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor The United States of America
References

Hampar B, Showalter SD. Non-transformed thymidine kinaseless cell line and its use for testing tumorigenic potential of genes. US Patent 4,465,769 dated Aug 14 1984

Hampar B, et al. Herpes simplex virus (type 1) thymidine kinase gene does not transform cells morphologically. Proc. Natl. Acad. Sci. USA 78: 2616-2619, 1981. PubMed: 6264486

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hampar B, Showalter SD. Non-transformed thymidine kinaseless cell line and its use for testing tumorigenic potential of genes. US Patent 4,465,769 dated Aug 14 1984

Hampar B, et al. Herpes simplex virus (type 1) thymidine kinase gene does not transform cells morphologically. Proc. Natl. Acad. Sci. USA 78: 2616-2619, 1981. PubMed: 6264486