ES-E14TG2a (ATCC® CRL-1821)

Organism: Mus musculus, mouse  /  Cell Type: embryonic stem cell , blastocyst derived  /  Tissue: embryo  / 

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Organism Mus musculus, mouse
Tissue
embryo
Cell Type embryonic stem cell , blastocyst derived
Product Format frozen
Morphology spherical
Culture Properties adherent
Biosafety Level 1
Age embryo, blastocyst embryo, blastocyst
Strain 129/Ola
Applications

This cell line is a suitable transfection host. 

They may also be of use in studies of early post implantation structures.
Storage Conditions liquid nitrogen vapor phase
Derivation
This is a derivative of one of several embryonal stem cell (ES) lines developed by M. Hooper in 1987.
Comments
The cells are deficient in HGPRT (HPRT) and are resistant to 0.06 mM 6-thioguanine.
The cells remain undifferentiated when cultured on feeder layers (embryonic fibroblasts or STO cells).
The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
When injected into blastocysts, the cells can colonize the germline.
After conventional molecular gene modification techniques, they may be used to reconstitute mouse embryos.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.1 mM ; fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution. Allow the trypsin to loosen the cells, add fresh medium, aspirate and dispense into new flasks containing pre-plated mitomycin-C treated feeder cells (ATCC 56-X.2).
Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor T Doetschman
References

Smith AG, Hooper ML. Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9, 1987. PubMed: 3569655

Kuehn MR, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-298, 1987. PubMed: 3029599

Doetschman T, et al. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578, 1987. PubMed: 3683574

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Smith AG, Hooper ML. Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9, 1987. PubMed: 3569655

Kuehn MR, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-298, 1987. PubMed: 3029599

Doetschman T, et al. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578, 1987. PubMed: 3683574